Multiple sequence alignment on the protein data set.

<p>Multiple sequence alignment was performed on 86 proteins in the data set using COBALT [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117874#pone.0117874.ref055" target="_blank">55</a>]. The resulting phylogenetic tree is visualized using Interactive tree of life (ITOL) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117874#pone.0117874.ref056" target="_blank">56</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117874#pone.0117874.ref057" target="_blank">57</a>] (Blue: PBPs, Green: Ambler Class A, Dark Blue: Ambler Class C, Yellow: Ambler Class D, Orange: Ambler Class B. Same coloring scheme is used in the next figure.)</p>