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Multi unit activities (MUA) at CA1 pyramidal cell layer upon optogenetic activation.

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posted on 2015-03-20, 03:25 authored by Norio Takata, Keitaro Yoshida, Yuji Komaki, Ming Xu, Yuki Sakai, Keigo Hikishima, Masaru Mimura, Hideyuki Okano, Kenji F. Tanaka

A, A photograph of a custom-made optrode, which was inserted vertically to record MUA at CA1 pyramidal cell layer of dHP of an anesthetized transgenic mouse. An expanded view of a tip of an optrode is shown in a circle at the right. A tungsten-wire was protruded ∼0.5 mm from a tip of an optical fiber, which corresponds to the distance between a tip of an optical fiber and illumination target in the hippocampus in fMRI experiments (see Fig. 1 B,C; S1 Fig.). Also note that a side of an optical fiber was painted in black to reduce stray light. Scale bar: 3.5 mm for a whole view, 1 mm for an expanded view. B, A representative time course of MUA activities. Vertical lines of blue and yellow indicate 0.5 s-illumination of light of each color, respectively. B2 and B3 show an expanded view of MUA upon blue and yellow light illumination, respectively. Note that in B1, vertical lines in blue and yellow are drawn thicker than 0.5 s for visibility purpose. C, MUA count per 1.5 s bin was obtained from 6 recording sessions from 3 transgenic animals. The x-axis at the top corresponds to fMRI scan counts. Gray shading indicates the SEM. Vertical lines in blue and yellow indicate bins when light in each color was illuminated at dHP. D, Mean traces of MUA during pre-stimulus period (0∼11 s) and the first activated period (11∼41 s) are shown in black and blue, respectively, obtained from 6 recording sessions from 3 transgenic animals.

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