Morphology and threshold of large-field RGCs.

(A) Top and side skeletal view of the On and Off large-field RGCs. Triangles indicate axons. Boxed regions indicate AIS. These cells were traced from mice retinas. (B) Comparison of the model neurons’ AIS thresholds to experimental results under equivalent conditions. The disk electrode was 40 µm axially above the cells’ somatic center. (C) Illustration of the threshold mapping procedure. A 10 µm radius disk electrode was moved through a 2D plane 40 µm axially above the somatic center. (D1) Threshold map of the Off RGC and expanded view of the dendro-somatic region (D2) and axo-somatic region (D3). (E1) Threshold map of the On RGC and expanded view of the dendro-somatic region (E2) and axo-somatic region (E3). Number next to each+symbol represents the threshold (in µA) at the marked location.