Morphological abnormalities of the mutant NP at E15.5.

<p><b>A</b>. H&E staining of E15.5 NP in control (Foxa2<sup>iCre</sup>;HIF-1α<sup>f/+</sup>) (a) and mutant (Foxa2<sup>iCre</sup>;HIF-1α<sup>f/f</sup>) (b) mice. Bar = 50 µm. <b>B</b>. Safranin O staining of E15.5 NP in control (HIF-1α<sup>f/f</sup>) (a) and mutant (Foxa2<sup>iCre</sup>;HIF-1α<sup>f/f</sup>) (b) specimens. Bar = 50 µm. <b>C</b>. In <i>situ</i> hybridization for aggrecan (a,b), collagen II (c,d), collagen X (e,f), and brachyury (g,h) mRNAs in control (HIF-1α<sup>f/f</sup>) (a,c,e,g) and mutant (Foxa2<sup>iCre</sup>;HIF-1α<sup>f/f</sup>) (b,d,f,h) NP at E15.5. Brightfield (a-f) and darkfield (g,h) pictures are shown. Bar = 50 µm.</p>