Models of WT and mutants reveal differences in the interface between linker and TI loop.

Representative snapshots showing the linker-TI loop interface of (A) the WT, (B) NEUT, (C) ALA, and (D) D400A model. For clarity, only K252, R253, Y255, D400X, E402X (X denotes the inserted mutation residue, Fig. 1C) and F401 are shown as indicated in ball-and-stick representation. Furthermore, the backbones of the linker and the TI loop are represented as blue and beige cartoon, respectively. For a better orientation, the membrane lipids are shown as cyan sticks. For the NEUT model, PI(4,5)P₂ molecules interacting with R253 are indicated, with C atoms in black.