Mitochondrial Activity. A

and B. %MTT activity as mitochondrial electron flow and UV auto-fluorescence of mitochondria as reduced pyridine nucleotides [NAD(P)H] respectively. C. Mitochondrial membrane potential was assessed using JC-1dye on flow cytometer. In dot plot, red fluorescence of J-aggregates represents polarized mitochondria (in right panel, red colored) whereas green fluorescence of JC-1 monomers represents depolarized mitochondria (left panel, green colored). CCCP (mitochondrial uncoupler) was used as negative control for mitochondrial membrane potential. Level of significance is denoted as **/## P<0.01 and ***/### P<0.001. *, compared to vehicle control and #, compared to nimesulide stress.