Midpoint rooted Neighbor-joining tree for the 76 L. donovani complex strains analysed.

This midpoint rooted tree was inferred from Dps-distances calculated for the MLMT data (14 microsatellite markers) of our sample set. The tree leaves are coloured according to the populations defined by structure analysis (Fig. 2). Bootstrap values (1000 re-samplings) above 50% are indicated at key nodes. The EP59 strain was excluded from this analysis due to its hybrid profile.