Figure_1.tif (807.1 kB)
Mct1 is apparent in cytoplasmic vesicles.
figure
posted on 2014-01-16, 02:48 authored by Amy L. Uhernik, Lun Li, Nathan LaVoy, Micah J. Velasquez, Jeffrey P. SmithRBE4 cells expressing mCherry-Mct1 were imaged live using confocal microscopy (A). In the last three micrographs, RBE4 cells were immunostained with an anti-Mct1 antibody, fixed in 3.7% formaldehyde, and permeabilized with either methanol and acetone (B), or 0.5% TritonX-100 (C), or 5% glacial acetic acid (D). Scale = 5 µM.