Matching peptides of AOx corresponding to conserved internal amino acid sequence as predicted by FindPept pmf search for tryptic digest of 2-D gel spot 6.

The pmf peak list (m/z values) generated from spot 6 of 2D gel was manually uploaded on FindPept online tool. Detectable m/z values were matched with the theoretical peptide masses generated from the virtual tryptic digest of query amino acid sequence of AOx from A.terreus NIH2624 strain as input. The output result shows user mass (practical), database mass (theoretical), delta mass (theoretical minus practical mass), peptide sequence and corresponding position in query sequence. The above table highlights only those two peptide fragments which correspond to conserved internal amino acid blocks from multiple sequence alignment of similar AOx from other filamentous fungi and yeast species.