Manganese pretreatment offers no protection against the effects of Stx in Vero cells.

One hundred percent protein synthesis corresponds to cells incubated with no exogenous MnCl₂ and no toxin. All points were assayed in triplicate and error bars indicate standard deviation. (A, B) Percent protein synthesis of Vero cells incubated in the presence or absence of 250 µM MnCl₂ for 4 hours followed by an additional 4 hour incubation at 125 µM MnCl₂ with increasing concentrations of either (A) Stx1-S or (B) Stx2a.