Figure_2.tif (3.27 MB)

Male Tysnd1^−/− mice are infertile.

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posted on 2013-02-14, 02:25 authored by Yumi Mizuno, Yuichi Ninomiya, Yutaka Nakachi, Mioko Iseki, Hiroyasu Iwasa, Masumi Akita, Tohru Tsukui, Nobuyuki Shimozawa, Chizuru Ito, Kiyotaka Toshimori, Megumi Nishimukai, Hiroshi Hara, Ryouta Maeba, Tomoki Okazaki, Ali Nasser Ali Alodaib, Mohammed Al Amoudi, Minnie Jacob, Fowzan S. Alkuraya, Yasushi Horai, Mitsuhiro Watanabe, Hiromi Motegi, Shigeharu Wakana, Tetsuo Noda, Igor V. Kurochkin, Yosuke Mizuno, Christian Schönbach, Yasushi Okazaki

A. Semi-thin (8–10 micron) testes sections of 20 weeks old Tysnd1^+/+ and Tysnd1^−/− mice were stained with hematoxylin-eosin. Abnormal, round sperm heads are visible in the seminiferous tubules of Tysnd1^−/− mice. B. Abnormal morphology of Tysnd1^−/− sperms. Epididymal sperms of 15 weeks-old Tysnd1^+/+ and Tysnd1^−/− mice were stained with mitochondrial stains (MitoFluor Red) and nuclear staining (DAPI). Scale bar = 20 µm. C. Percentage of sperms showing normal morphology in Tysnd1^+/+ (n = 4), Tysnd1^+/− (n = 4) and Tysnd1^−/− (n = 5) mice. Each error bar represents the mean ± SE. D. Anti-MN9 antibody immunostaining and Hoechst nuclear staining of epididymal sperms isolated from a 20 weeks old Tysnd1^−/− mouse (red: acrosome and blue: nucleus). Arrow heads and arrows indicate abnormal round-headed sperms and normal sperms, respectively. Scale bar = 5 µm. E. Acrosomes of a semi-thin testis section from a 10 weeks old Tysnd1^−/− and a heterozygous control mouse were stained with PNA-FITC (green) and Hoechst nuclear stain (blue). F. EM image of a Tysnd1^−/− caudal epididymal sperm. The round-headed sperm lacks the acrosome and shows an abnormal mitochondrial sheath (M) around the nucleus (N). Scale bar = 1 µm. G. EM image showing normal spermatogenesis in Tysnd1^+/− male mice. S7: step 7 round spermatid; S16: step 16 elongated spermatid. Acrosomes (A) are normally formed. Scale bar = 2 µm. H. EM image of Tysnd1^−/− elongated spermatid. S16: step 16 spermatid. In some spermatids the acrosome (*) is detached from the nucleus (N). Scale bar = 2 µm.