Maize HD-Zip II proteins function as transcriptional repressors and ATHB17Δ113 relieves the repression caused by endogenous maize HD-Zip II proteins.
(A) Cells were co-transformed with 4 µg/320,000 cells of each reporter (right side label) and an increasing amount of each HD-Zip II family member (left-to-right for each color: 0.008; 0.04; 0.2; 1; 5 µg/320,000 cells). Error bars are 1 SD. (B) Cells were triple-transformed with 4 µg/320,000 cells of each reporter (right side label), 20 ng of each HD-Zip family member and an increasing amount of ATHB17Δ113 (left-to-right for each color: 0.0, 0.3; 0.6; 1.3; 2.5 µg/320,000 cells). For a given reporter, HD-Zip II, and concentration of ATHB17Δ113, the calculation of standard error relies on the number of biological replicates and the estimated error variance derived from a one-way ANOVA.