Fig_7.tif (4.4 MB)
MPV17 loss of function affects the purine branch of mitochondrial salvage pathway.
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posted on 2016-01-18, 15:33 authored by Ilaria Dalla Rosa, Yolanda Cámara, Romina Durigon, Chloe F. Moss, Sara Vidoni, Gokhan Akman, Lilian Hunt, Mark A. Johnson, Sarah Grocott, Liya Wang, David R. Thorburn, Michio Hirano, Joanna Poulton, Robert W. Taylor, Greg Elgar, Ramon Martí, Peter Voshol, Ian J. Holt, Antonella SpinazzolaRepresentative immunoblot thymidine kinase 2 (TK2) in (A) control and MPV17-mutant fibroblasts in dividing and quiescent cells, and (B) in the liver of wild-type (WT) and knockout (KO) mice. (C) Steady state levels of adenylate kinase 2 and 3 (AK2 and AK3) and Deoxyguanosine Kinase (Dguok) in the liver of wild-type (WT) and knockout (KO) mice. The arrow indicates the Dguok isoform downregulated in KO mouse liver. The samples were from 2 month-old mice unless indicated. (D) AK2, AK3 and DGUOK steady state levels in control and MPV17 mutant fibroblasts in proliferating and quiescent cells. Vinculin, GAPDH, and Tom20 were used as loading control.
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dNTP levelsMitochondria MPV 17mitochondrial DNA lossMPV 17 deficiencymitochondrial DNA depletiondysfunction causes mitochondrial DNA abnormalitiesMPV 17 Loss Causes Deoxynucleotide Insufficiencydeoxynucleoside supplementationSlow DNA Replicationmitochondrial deoxynucleotide homeostasismitochondrial genomic instabilityDNA copy numberMPV 17 disease models
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