MIC values and kinetic and stability parameters of TEM-1, single mutants R164S and G238S, and double mutant R164S/G238S.

<p>The kinetic were acquired <i>in vitro</i> with purified proteins. The <i>T<sub>m</sub></i> and <i>m</i> values (indicating the cooperativity of unfolding) were extracted from heat denaturation. N.D.; not determined. Errors shown are standard deviations. Because of the high TEM-1 levels under induction, MIC assays for ampicillin were determined without the addition of IPTG. For additional details see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1001321#pgen.1001321.s002" target="_blank">Figures S2</a> and <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1001321#pgen.1001321.s003" target="_blank">S3</a>.</p>a<p>Median value across three replicates (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1001321#pgen.1001321.s006" target="_blank">Table S3</a> for replicate measurements).</p>b<p>The error ranges for TEM-1 and mutants R164S and R164S/G238S were determined from two independent measurements with two different protein preparations; error ranges for the remaining variant are estimated to be within the same range.</p>