MFSV transcript accumulation in <i>G. nigrifrons</i> transmitters and acquirers.

<p>Accumulation of MFSV transcripts relative to the N gene was determined using RT-qPCR. Transcript accumulation was calculated according to the 2<sup>−ΔΔCt</sup> algorithm using the <i>ribosomal protein S13</i> (RPS13) gene expression as the calibrator. Means for relative accumulation of each gene are shown. Bars with different letters were significantly different using LSD (<i>P</i><0.05).</p>