MCM4 expression in <i>Leishmania</i>.

<p><b>A</b>. Schematic representation of the conserved domains in LdMCM4. PIP box - PCNA interacting protein motif; A – Walker A motif; B – Walker B motif; R – arginine finger; Z – zinc finger; S1 – sensor 1; S2 – sensor 2. <b>B</b>. SDS-PAGE (10% PAGE) analysis of overexpressed and purified recombinant LdMCM4 (2 ug; ∼97.2 kDa). <b>C</b>. Western blot analysis of recombinant LdMCM4 with mouse anti-MCM4 antibody (1∶5000 dilution). <b>D</b>. Western blot analysis (10% SDS-PAGE) using anti-MCM4 antibodies (dilution of 1∶1000). First panel - lane 1, recombinant MCM4; lane 2, <i>L. donovani</i> whole cell extracts. Second panel - Whole cell extracts from <i>Leishmania</i> promastigotes and amastigotes (4×10<sup>7</sup> cell equivalents) probed with anti-MCM4 antibodies and anti-tubulin antibodies (Zymed Laboratories; 1∶2000 dilution; loading control). <b>E</b>. Western blot analysis (10% SDS-PAGE) of whole cell extracts from <i>Leishmania</i> procyclics and metacyclics (4×10<sup>7</sup> cell equivalents) probed with anti-MCM4 antibodies and anti-tubulin antibodies (loading control).</p>