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MALP-2 induces PI3K activation is regulated by the formation of a Btk, Mal, c-Src and p85α complex.

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posted on 2014-07-31, 03:00 authored by Xiaoxing You, Liangzhuan Liu, Yanhua Zeng, Ranhui Li, Jun He, Xiaohua Ma, Chuanhao Jiang, Cuiming Zhu, Liesong Chen, Minjun Yu, Guangli Ou, Yimou Wu

A. Cells were pretreated with or without LFM-A13 (100 µM), or PP1 (50 µM) for 1 h, and then incubated in the absence or presence of 5 ng/ml MALP-2 for 15 min. Cell fractions were prepared and subjected to Western blotting analysis with an anti-p-Akt antibody. Data shown represents three experiments (means ± SEM). *, P<0.05 and **, P<0.01 for significant difference between compared groups. B, C. Cells were transfected with siRNA of Mal or MyD88, and then treated with MALP-2 for 15, 60 or 120 min. Phosphorylated Akt was detected by Western blotting. *, P<0.05 for significant difference between compared groups. D. Cells were treated with MALP-2 for the indicated times, the cell lysates were subjected to immunoprecipitation using an anti-c-Src or anti-p85α antibody, and followed by immunoblotting with anti-Btk, anti-Mal, anti-MyD88, anti-c-Src or anti p85α antibody. All figures are representative of three independent experiments.

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