Figure_3.tif (243.71 kB)
Lucanthone/Hycanthone promote APE1 cleavage at lower concentration than CRT.
figure
posted on 2011-09-15, 01:18 authored by Mamta D. Naidu, Rakhi Agarwal, Louis A. Pena, Luis Cunha, Mihaly Mezei, Min Shen, David M. Wilson III, Yuan Liu, Zina Sanchez, Pankaj Chaudhary, Samuel H. Wilson, Michael J. WaringWestern blot of APE1 over expresser clone 5 pretreated with increasing concentration of lucanthone (2.5–100 µM) and CRT0044876 (2.5–200 µM) in presence of protease inhibitor cocktail for 2 h at 37°C (10 µg of total cell protein loaded per lane). The corresponding decrease in 35.5 kDa APE1 protein band and increase in 25-kDa-degradation product is indicated by the arrows.
