<i>Loxosceles</i> and <i>Sicarius ornatus</i> venom SMases D incorporate into human erythrocytes and cause loss of glycophorin C expression.

<p>Erythrocytes were treated with venoms from <i>Sicarius ornatus</i> or <i>Loxosceles</i> or with VBS<sup>2+</sup> buffer (control) and analyzed for the expression GPC by flow cytometry [A]. The ability of the toxins to insert into the erythrocyte surface was analyzed using a monospecific polyclonal rabbit serum against <i>Loxosceles intermedia</i> SMases D [B]. Results are representative for three different experiments and expressed as median of fluorescence of duplicates ± SD. The variation among experiments was around 10%. (*) Significant differences (<i>P</i><0.05) from control; (#) Significant differences from <i>L. laeta</i> venom (<i>P</i><0.05); (•) Significant difference between female and male <i>Sicarius</i> venoms (<i>P</i><0.05).</p>