Lower level of aquaporin-4 (AQP4) immunoreactivity in the LBP-treated I/R retina.

(A–C) Photomicrographs taken with a 40x objective. (D–F) Photomicrographs taken with a 60x objective. In the non-ischemic control retina, mild AQP4 immunoreactivity was observed in the astrocytic end-feet in ILM and perivascular regions near INL (A & D). However, intense AQP4 immunoreactivity was seen in the astrocytic end-feet in ILM and perivascular regions of the vehicle-treated I/R retina (B & E). However, the AQP4 immunoreactivity in LBP-treated I/R retina was comparable with that in the non-ischemic control retina (C & F). Results were further confirmed by the semi-quantitative analysis (G). ^##p<0.01 vs. non-ischemic control retina, *p<0.05 vs. vehicle-treated I/R retina. Scale bar for A–C, 25 µm. Scale bar for D–F, 15 µm.