Loss of G9a does not affect viral loads upon DCV infection.

(A,B) Wild-type or G9a mutant flies were inoculated with DCV and viral titers were determined over time in (A) whole flies, and (B) dissected fat bodies. Data represent means and s.d of three independent experiments. Each experiment contained three biological replicates of 5 female flies (A), or 10 fat bodies (B) per replicate for each genotype. (C,D) DCV RNA levels over the course of 3 days post-infection analyzed by RT-qPCR in (C) whole flies or (D) fat bodies of wild-type and G9a mutant flies. DCV RNA levels were normalized to transcript levels of the housekeeping gene Ribosomal Protein 49 and are calculated relative to the viral RNA levels in flies harvested immediately after inoculation (t₀). Data represent means and s.d. of three biological replicates of 5 female flies (C) or 10 fat bodies (D) per replicate for each genotype. Data in panel C and D are from one experiment representative of 2 independent experiments. *P < 0.05 (Student’s t-test).