Loss of CLPTM1L sensitizes lung tumor cells to genotoxic agents.

A) Knockdown of CLPTM1L expression in A549 cells via stable shRNA. Confirmation of knockdown by western blot (inset). B) Knockdown of CLPTM1L expression in H838 cells via stable shRNA. Confirmation of knockdown by western blot (inset). C) Relative cell viability of A549 cells with CLPTM1L knockdown after 48 hours treatment with media or cisplatin. D) Relative cell viability of H838 cells with CLPTM1L knockdown after 48 hours treatment with DMSO vehicle or 1 µM camptothecin. E) Relative cell viability of H1299 cells with CLPTM1L over-expression after 48 hours treatment with DMSO vehicle or 10 µM camptothecin. Error bars represent one standard deviation from the mean of biological triplicates.