Long-term ISA27 treatment of U87MG cells.

<p>U87MG cells were treated with 2.5 µM ISA27 or 10 µM Nutlin-3 for 24, 48, 72, 96 and 120 h. The figure shows the viable cells that were counted by Trypan blue exclusion at the indicated times. The percentage of ISA27- (<b>▾</b>) or Nutlin-3- (<b>▪</b>) treated viable cells was calculated with respect to untreated viable cells at the indicated incubation times. Points, means of three independent experiments performed in duplicate; bars, SEM.</p>