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Long-term ISA27 treatment of U87MG cells.

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posted on 2013-08-19, 03:55 authored by Barbara Costa, Sara Bendinelli, Pamela Gabelloni, Eleonora Da Pozzo, Simona Daniele, Fabrizio Scatena, Renato Vanacore, Pietro Campiglia, Alessia Bertamino, Isabel Gomez-Monterrey, Daniela Sorriento, Carmine Del Giudice, Guido Iaccarino, Ettore Novellino, Claudia Martini

U87MG cells were treated with 2.5 µM ISA27 or 10 µM Nutlin-3 for 24, 48, 72, 96 and 120 h. The figure shows the viable cells that were counted by Trypan blue exclusion at the indicated times. The percentage of ISA27- () or Nutlin-3- () treated viable cells was calculated with respect to untreated viable cells at the indicated incubation times. Points, means of three independent experiments performed in duplicate; bars, SEM.

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