Long-term ISA27 treatment induces cell cycle arrest and a persistent increase in p21 mRNA levels in U87MG cells.

<p><i>7A) Flow cytometric cell cycle profiling</i>: the figure shows the percentage of untreated and MDM2 inhibitor-treated U87MG cells in G1-, S- and G2/M-phase. <i>7B) p21 mRNA evaluation</i>: ISA27 treatment resulted in a statistically significant increase in p21 mRNA levels at 24, 48 and 72 h.</p>