Localization of HeT-A Gag-FLAG proteins in S2 cells by confocal microscopy.

Stable S2 cells expressing wild and mutant (M1, M2, and M3) HeT-A Gag-FLAG protein were induced with CuSO₄, plated on a cover slip and stained with anti-FLAG antibody. Untransfected S2 cells were used as control. In all four cells lines tested HeT-A Gag was localized to the nucleus.