Lipophilic fluorescent dye (DiD) transfer depends on TraA and cell motility.

<p>Column headers list relevant properties of isogenic recipients. The same nonmotile (DK8601) donor was used in all mixtures and recipients were DW1414 (A<sup>+</sup> <i>traA<sup>+</sup></i>), DW1416 (A<sup>+</sup> <i>traA<sup>−</sup></i>) and DK8606 (A<sup>−</sup> <i>traA<sup>+</sup></i>). The ability of the DiD dye (red fluorescence) to be transferred to GFP recipients was assessed in merged panels (100× objective). Here yellow color indicates DiD transfer to GFP recipients and representative cells are noted by arrows.</p>