Light micrographs of mouse renal cortical tissues with HE staining.

The tissues were prepared by in vivo cryotechnique (IVCT) under normotensive condition (A) and acute hypertensive (B), and cardiac arrest (C) condition, as well as resection kidney tissue processed by immersion-fixation methods under normotensive condition (D). Under the acute hypertensive condition, the Bowman's space and luminal spaces of the proximal and distal tubules were widely open (B), but not in the other two IVCT groups (A,C). Scale bars  = 20 μm.