Levels of transcript abundance from genes involved in quinate metabolism.

A. Heatmap showing levels of transcript abundance during time course of appressorium development. Levels of expression are represented as moderated log2 ratio of transcript abundance as compared to mycelium grown in complete medium. Values are from 4 h (T4) to 16 h (T16) after conidia were incubated on a hydrophobic surface. Genes showing similar patterns of gene expression have been clustered. Homologues of genes involved in quinate metabolism in Neurospora crassa are labelled: quinate activator (qa-1F), quinate repressor (qa-1S), 3-dehydroquinase (qa-2), Quinate dehydrogenase (qa-3), 3-dehydroshikimate dehydratase (qa-4), function unknown (qa-x), quinate permeases (QP), shikimate pathway (S). B. Expression of genes involved in quinate metabolism. For each enzyme, bar graphs show abundance of transcripts encoding the gene in: Guy11 mycelium grown in complete medium (green bar), Δpmk1 mutant conidia left to germinate for 4 h (red bar), a Guy11 time course of appressorium development (4 h, 6 h, 8 h, 14 h, 16 h – black bars left to right).