Lentivirus-mediated miR-223 over-expression in HeLa cells.

(A) Lentivirus vector pLL3.7-miR-223 was packaged in HEK-293T cells which was less 20 generations. The packaging efficiency was evaluated by GFP fluorescent signal. Original magnification 10 × (B) HeLa cells were infected with the Lentivirus which was packaged and prepared from HEK-293T cells. The GFP fluorescent signal could be stably observed 72 hours after infection. EV group stood for vector control at the left and pLL3.7-miR-223 infection group was at the right. Original magnification 20 × (C) The infected HeLa cells were sorted by FACS. The fluorescence-positive cells were collected in either EV control (left) or miR-223 (right) group. Original magnification 10× (D,E) Mir-223 was over-expressed in HeLa cells and confirmed by both stem-loop RT-PCR (D) and quantitative PCR (E). NV: no virus control, EV: empty virus vector control, Input: positive mature miR-223.