Lentiviral vector delivery of hIL-7 promotes homeostatic proliferation of adoptively transferred human T cells in Rag2-/-γc-/- mice.
2010-08-06T02:02:57Z (GMT) by
<p><b>a.</b> Serum concentrations of hIL-7 detected by ELISA three weeks after intravenous administration of 9×10<sup>7</sup> or 1.7×10<sup>8</sup> IU of lentivirus expressing either luciferase or hIL-7. <b>b.</b> The percentage of CD3+, CD4+ or CD8+ T cells of live splenocytes following one week post transfer of 2×10<sup>7</sup> CFSE labeled human PBMCs into Rag2-/-γc-/- mice from A. <b>c.</b> Average mean fluorescence intensity (MFI) of CFSE measured by flow cytometry in T-cell subsets quantified in B. Four mice were used per group, and the average and SEM are shown. <b>d.</b> Representative histograms showing CFSE loss by CD3+, CD4+ or CD8+ adoptively transferred T cells from mice receiving the control vector, low dose hIL-7 or high dose hIL-7.</p>