Length histograms of the Tritonia Trinity-assembled transcripts and TransDecoder-predicted peptide sequences.

A) The length distribution of contigs (black bars) and longest contig per component (LCPC; red bars) reveals that a major portion of Trinity-assembled sequences range between 200 to 399 nucleotides in length, and that transcript and LCPC counts drop rapidly with increasing length. B) Length histogram of longest reading frames predicted from Trinity transcripts using TransDecoder. The bars are partitioned to show the percentage of open reading frames (ORFs) that are complete (black bars), missing the 5’ end (cyan), missing the 3’ end (red), or missing both 5’ and 3’ ends (orange bars). The percentage of complete ORFs increases with length, where only 36% are complete with lengths between 99 and 149 amino acids, whereas 55% are complete above 1,250 amino acids with a peak of 66% between 1,100 and 1,149 amino acids (red text).