LY294002, wortmannin and rapamycin attenuates flagellin-induced cell proliferation.

Ana-1 cells were pretreated with LY294002 (10 μM), wortmannin (10 nM) and rapamycin (100 nM) for 4 h and then stimulated with 100 ng/ml flagellin for 24 h, and cell proliferation was measured by methyl thiazolyl tetrazolium (MTT) assay. A. Proliferation was inhibited by LY294002 and wortmannin in response to flagellin. B. Proliferation was inhibited by rapamycin in response to flagellin. Experiments were made in triplicate, and data are representative of three separate experiments (mean ± SD). *p< 0.05 compare to untreated controls, # p< 0.05 compared with flagellin group.