LRP-1-silencing increases adhesion rate of carcinoma cells through JNK hyperactivation.
shCTRL (white boxes) and shLRP-1 (grey boxes) cells were seeded onto gelatin-coated plates and the non adherent cells were discarded after 30, 60 or 90 min. Adhesion assays were performed with shCTRL cells overexpressing wild type MKK-7/JNK1 (A) and shLRP-1 overexpressing a dominant negative mutant of JNK-1 (dn-JNK) (B) kinases. For each condition, results are expressed as percentages of corresponding control adherent cells at 90 minutes. Each value is the mean +/− SD for four separate experiments, with each performed in triplicate. *, P<0.05.