LPS induced neutrophil inflammasome activation depends on ROS production and lysosome destabilization.

2012-04-05T02:03:14Z (GMT) by Rongze Lu Hao Pan John E. Shively
<p>(<b>A</b>) IL-1β production in the supernatants of wild type (WT), P2X7<sup>−/−</sup> and Cybb<sup>−/−</sup> neutrophils under different treatment conditions: LPS (100 ng/ml), KN62 (2 µM, neutrophils were pre-treated for 30 minutes before LPS treatment), APDC (100 µM), bafilomycin A (125 nM), z-YVAD-fmk (1 mM). (<b>B</b>) Immunoblot analysis showing caspase-1 activation of WT, P2X7<sup>−/−</sup> and Cybb<sup>−/−</sup> neutrophils under different treatment conditions. (<b>C</b>) ROS production by LPS treated WT and CEACAM1<sup>−/−</sup> neutrophils with or without APDC (100 µM) as measured by MFI of fluorescent probe H2DCFDA using FACS. Data are representative of 3 different experiments (<b>B</b>) and p values (<b>A</b> and <b>C</b>) were calculated by a 2-tailed T-test, **0.001</p>