LPS induced neutrophil inflammasome activation depends on ROS production and lysosome destabilization.
(A) IL-1β production in the supernatants of wild type (WT), P2X7−/− and Cybb−/− neutrophils under different treatment conditions: LPS (100 ng/ml), KN62 (2 µM, neutrophils were pre-treated for 30 minutes before LPS treatment), APDC (100 µM), bafilomycin A (125 nM), z-YVAD-fmk (1 mM). (B) Immunoblot analysis showing caspase-1 activation of WT, P2X7−/− and Cybb−/− neutrophils under different treatment conditions. (C) ROS production by LPS treated WT and CEACAM1−/− neutrophils with or without APDC (100 µM) as measured by MFI of fluorescent probe H2DCFDA using FACS. Data are representative of 3 different experiments (B) and p values (A and C) were calculated by a 2-tailed T-test, **0.001