Knockdown of SKN-1 or TEFs, but not TIFs, reduces UPS activity in the intestine.
2011-06-09T01:53:33Z (GMT) by
<p>(A) Representative images of <i>Pvha-6::UbG76V-Dendra2 and Pvha-6::Dendra2</i> reporters in animals exposed to control, <i>skn-1</i>, <i>eef-1A.1</i> (TEF) or <i>eif-1</i>(TIF) RNAi. Red fluorescence derives from substrate that was present at the time of photoconversion. Bar: 20 µm. In (A–C), bar graphs depict the percentages of green and red fluorescence compared to either the initial value (t = 0 for green) or point of photoconversion (t = C for red) for UbG76V-Dendra2 and Dendra2, respectively (± SEM). (B,C) Summary of UbG76V-Dendra2 and Dendra2 imaging in intestinal cells after 9 hours in response to TEF and TIF RNAi, respectively. Significant differences relative to control RNAi (red fluorescence after 9 hours) are indicated with **<i>P</i><0.01, *<i>P</i><0.05.</p>