Knockdown of JARID2 affected the TGF-ß-dependent changes in expression of EMT-related genes in A549 cells.

QRT-PCR analysis was performed to detect the expression of CDH1/E-cadherin (A), FN1/Fibronectin (B), Vimentin (C), ZEB1 (D), ZEB2 (E), miR-200a (F), miR-200c (G) and JARID1B (H) in A549 cells infected with retroviruses expressing control shRNA or JARID2 shRNA with or without the treatment of 1 ng/ml of TGF-ß for 24 hours (*, P<0.01 comparing to control). (I) Western blot was performed to detect the expression of E-cadherin, Fibronectin, Vimentin, ZEB1, ZEB2, phosphorylated SMAD3 (P-SMAD3) and GAPDH proteins using the corresponding antibodies.