Knockdown of <i>JARID2</i> affected the TGF-ß-dependent changes in expression of EMT-related genes in A549 cells.

<p>QRT-PCR analysis was performed to detect the expression of <i>CDH1/E-cadherin</i> (A), <i>FN1/Fibronectin</i> (B), <i>Vimentin</i> (C), <i>ZEB1</i> (D), <i>ZEB2</i> (E), <i>miR-200a</i> (F), <i>miR-200c</i> (G) and <i>JARID1B</i> (H) in A549 cells infected with retroviruses expressing control shRNA or <i>JARID2</i> shRNA with or without the treatment of 1 ng/ml of TGF-ß for 24 hours (*, <i>P</i><0.01 comparing to control). (I) Western blot was performed to detect the expression of E-cadherin, Fibronectin, Vimentin, ZEB1, ZEB2, phosphorylated SMAD3 (P-SMAD3) and GAPDH proteins using the corresponding antibodies.</p>