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Kinetics of FXa and Elastase interactions with rSimukunin.

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posted on 2012-02-23, 01:53 authored by Hitoshi Tsujimoto, Michail Kotsyfakis, Ivo M. B. Francischetti, Jai Hoon Eum, Michael R. Strand, Donald E. Champagne

Responses were obtained by injecting FXa or elastase over immobilized rSimukunin for 180 seconds at a flow rate of 30 µl/minute. Data were derived from Ka1 and Kd1 and fitted using the Langmuir (1∶1 binding) equation. Kinetic values obtained from the sensorgrams presented in Figure 6B and 6C for FXa and elastase respectively.

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