Kinetic analysis of leukocyte migration in the airways in bleomycin treated mice.
Leukocytes from BAL fluid were collected on day 0, 1, 3, 5, 10 and 21 after intra-nasal injection of bleomycin (7.5 mg/kg) in WT balb/c mice. A) The absolute number of leukocytes in BAL fluid was determined by flow cytometry at the indicated time points. B) The number of macrophages and neutrophils were determined by flow cytometry using specific antibodies against macrophages (F4/80) and neutrophils (Ly6G). C) The number of T cells (TCRβ+), B cells (CD19+), and NK cells (DX5+TCRβ-) were measured in BAL fluid by flow cytometry. n = 3–8 mice per time point. *p<0.05, ** p<0.01, by ANOVA followed by t-test, comparing indicated time points post-bleomycin injection to t = 0.