JNK independent cJun phosphorylation.
2011-04-29T02:39:53Z (GMT) by
<p>Purified B lymphocytes were stimulated through both CD40 and TLR7 for indicated times. Cells were lysed and analyzed by Western blot for A) phospho–cJun, total -cJun and Actin as a loading control and B) CDK4 and cyclin D2. C) Resting splenic B lymphocyets were stimulated through TLR7 and CD40 (R848 1 ug/ml and CD40L) for the indicated times. Cells were lysed and analyzed for phosphorylated MAP kinases by Western blot. D) Mouse high density splenic B cells or E) human peripheral B cells were stimulated through both CD40 and TLR7 for the designated times in the absence or presence of CDK4 inhibitors SU9516 – CDK-I (10 uM) or Fascaplysin (5 uM) for 5 hours. Cells were then lysed and analyzed for phospho-cJun by Western blot. Results are representative of >3 separate experiments.</p>