Intracellular interferon-γ production in patients with anti-IFN-γ AAbs and healthy subjects.

PBMCs were stimulated with immobilized anti-CD3 mAb. The PBMCs were then stained surface CD3 using PerCP conjugated anti-CD3 mAb and PE conjugated anti-cytokine antibody. The expression of the intracellular IFN-γ of the CD3+ T cell was analyzed by flow cytometry. The fold increase in the IFN-γ production in response to the stimulants as compared to cell culture with no stimulants is shown. “**” represents p<0.005.