Intracellular cytokine production of patients with anti-IFN-γ AAbs and healthy subjects.

<p>PBMCs were stimulated with PMA and ionomycin (Iono). The PBMCs were then stained surface CD3 using PerCP conjugated anti-CD3 mAb and PE conjugated anti-cytokine antibody. The expression of the indicated intracellular cytokines of the CD3<sup>+</sup> T cell was analyzed by flow cytometry. The fold increase in the cytokine production in response to the stimulants as compared to cell culture with no stimulants is shown. “<i>ns</i> represents no statistical significance”; “**” represents <i>p<0</i>.<i>005</i>; “***” represents <i>p<0</i>.<i>001</i>.</p>