Intracellular cytokine production of patients with anti-IFN-γ AAbs and healthy subjects.

PBMCs were stimulated with PMA and ionomycin (Iono). The PBMCs were then stained surface CD3 using PerCP conjugated anti-CD3 mAb and PE conjugated anti-cytokine antibody. The expression of the indicated intracellular cytokines of the CD3+ T cell was analyzed by flow cytometry. The fold increase in the cytokine production in response to the stimulants as compared to cell culture with no stimulants is shown. “ns represents no statistical significance”; “**” represents p<0.005; “***” represents p<0.001.