Interaction of SP-R210 with innate immune receptors.

Immunoprecipitation experiments were carried out using 5 mg/mL (A and B) or 2.5 mg/mL (C) cell extracts from control and SP-R210L(DN) macrophages with polyclonal anti-SP-R210 (A) or monoclonal CD11b (B). To assess the effect of LPS treatment, immunoprecipitation reactions were carried out 10, 30, 60, and 120 min after treatment with 100 ng/mL LPS (C). Co-precipitated proteins were separated on SDS-PAGE gels and blotted with indicated antibodies. Extracts immunoprecipitated with monoclonal anti-SP-R210 were re-probed with polyclonal SP-R210 antibodies. A monoclonal IgG1 against an unrelated viral antigen served as control (C). Results shown are representative of 2–4 independent experiments.