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Interaction of Girdin with Par-3.

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posted on 2013-02-20, 05:32 authored by Kei Ohara, Atsushi Enomoto, Takuya Kato, Takahiko Hashimoto, Mayu Isotani-Sakakibara, Naoya Asai, Maki Ishida-Takagishi, Liang Weng, Masanori Nakayama, Takashi Watanabe, Katsuhiro Kato, Kozo Kaibuchi, Yoshiki Murakumo, Yoshiki Hirooka, Hidemi Goto, Masahide Takahashi

A. Interaction of exogenously expressed Girdin and Par-3 in HEK293T cells. Total cell lysates (TCL) from HEK293T cells that expressed Girdin-V5 and either myc-GST or myc-Par-3 were immunoprecipitated (IP) with anti-myc antibody, followed by Western blot analyses using the indicated antibodies. myc-Par-3 and Girdin-V5 are indicated by asterisks and stars, respectively. Open arrowhead indicates IgG heavy chain. B, C. Interaction between endogenous Girdin and Par-3. Lysates from HEK293T cells were immunoprecipitated with two anti-Girdin antibodies (B, available from IBL; C, available from R&D Systems) and normal rabbit IgG antibodies, followed by Western blot analysis using the indicated antibodies. Two spliced isoforms of Par-3 are indicated by arrowheads.

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girdin

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