Initial rate of DDT-induced aggregation of BSA as a function of α-crystallin concentration (45°C; 2 mM DTT).

The dependence of (K_agg/K_agg,0)^1/n on α-crystallin concentration (lower abscissa axis) or the ratio of the molar concentrations of α-crystallin and BSA (upper abscissa axis x = [α-crystallin]/[BSA]; n = 1.6). Points are the experimental data. The solid line in the interval of x values from 0 to x₁ = 0.17 was calculated from Eq. (12) at S₀ = 0.40 subunits of α-crystallin per one BSA molecule. The solid line in the interval of x values from x₁ = 0.17 to x₂ = 2.6 was calculated from Eq. (13) at Y₀ = 0.94 and x_0.5 = 0.093. The inset shows the dependence of the adsorption capacity (AC) of α-crystallin with respect to the target protein on x.