Initial rate of DDT-induced aggregation of BSA as a function of α-crystallin concentration (45°C; 2 mM DTT).

<p>The dependence of (<i>K</i><sub>agg</sub>/<i>K</i><sub>agg,0</sub>)<sup>1/<i>n</i></sup> on α-crystallin concentration (lower abscissa axis) or the ratio of the molar concentrations of α-crystallin and BSA (upper abscissa axis <i>x</i> = [α-crystallin]/[BSA]; <i>n</i> = 1.6). Points are the experimental data. The solid line in the interval of <i>x</i> values from 0 to <i>x</i><sub>1</sub> = 0.17 was calculated from Eq. (12) at <i>S</i><sub>0</sub> = 0.40 subunits of α-crystallin per one BSA molecule. The solid line in the interval of <i>x</i> values from <i>x</i><sub>1</sub> = 0.17 to <i>x</i><sub>2</sub> = 2.6 was calculated from Eq. (13) at <i>Y</i><sub>0</sub> = 0.94 and <i>x</i><sub>0.5</sub> = 0.093. The inset shows the dependence of the adsorption capacity (AC) of α-crystallin with respect to the target protein on <i>x</i>.</p>

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