Inhibitory Effect of Template DNA from FFPE Tissues on PCR Performance.
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posted on 2013-10-14, 02:53 authored by Dimo Dietrich, Barbara Uhl, Verena Sailer, Emily Eva Holmes, Maria Jung, Sebastian Meller, Glen KristiansenqPCR with 1 µg of genomic HMW template DNA and increasing amounts (60-1,440 ng) of spiked genomic template DNA from FFPE tissue Genomic DNA from FFPE tissue was treated beforehand with active DNase I (+) and heat-inactivated DNase I (-), respectively. qPCR was performed using a 150-bp fragment and 1 U Taq polymerase. Shown are the mean values (± standard deviations) from triplicate measurements.
