Inhibition of water permeability by mercury chloride (HgCl<sub>2</sub>) in yeast strains expressing <i>V. vinifera</i> aquaporins.

<p>(A) Inhibition of water permeability in yeast cells expressing functional aquaporin (<i>Vv</i>TnTIP2;2) by HgCl<sub>2</sub>. The green signal represents water permeability without HgCl<sub>2</sub> and the red signal shows the lower permeability when cells were incubated with 0.5 mM HgCl<sub>2</sub> for 15 minutes prior to osmotic shock. (B) Water permeability coefficients (<i>P<sub>f</sub></i> at 23°C) of <i>Vv</i>TnPIP2;1, <i>Vv</i>TnTIP1;1 and <i>Vv</i>TnTIP2;2 were reduced by 0.5 mM HgCl<sub>2</sub>. Data are mean ± SD of three independent experiments with at least five traces. The data were analyzed by <i>t-test</i> and asterisks above bars indicate statistically significant differences, where **p<0.01, ***p<0.001.</p>