Figure_5.tif (199.11 kB)
Inhibition of the ATPase activity of full-length NS3 by the compounds 9b and 9c.
figure
posted on 2013-12-20, 03:35 authored by Emmerson C. B. da Costa, Raquel Amorim, Fernando C. da Silva, David R. Rocha, Michelle P. Papa, Luciana B. de Arruda, Ronaldo Mohana-Borges, Vitor F. Ferreira, Amilcar Tanuri, Luciana J. da Costa, Sabrina B. FerreiraIncreasing concentrations of naphthoquinones 9b and 9c were tested for their activity against ATPase site. The reaction was performed in buffer containing 40 mM Tris-HCl (pH 7.5), 5 mM DTT, 100 mM KCl, and 5 mM MgCl2. The compounds were pre-incubated with 600nM NS3FL for 10 min at 30 °C followed by the addition of 1 mM ATP. Each point represents the average of three independent replicates in different concentrations of the compounds.
