figshare
Browse
Figure_5.tif (3.55 MB)

Inhibition of p-ERK with UO126 decreases Snail and reverts EMT independent of proteasomal degradation.

Download (0 kB)
figure
posted on 2014-08-14, 10:42 authored by Bethany N. Smith, Liza J. Burton, Veronica Henderson, Diandra D. Randle, Derrick J. Morton, Basil A. Smith, Latonia Taliaferro-Smith, Peri Nagappan, Clayton Yates, Majd Zayzafoon, Leland W. K. Chung, Valerie A. Odero-Marah

(A) Expression of p-ERK, ERK and Snail was analyzed by Western blot analysis in MCF-7 Neo and MCF-7 Snail cells treated with DMSO control (Ctrl) or UO126 for 30 min, 2 h, 6 h, and 24 h. (B) MCF-7 Neo and MC-7 Snail cells were treated with either DMSO (control) or UO126 and stained with DAPI. Cell morphology and integrity were analyzed by merging DAPI immunofluorescence imaging with brightfield microscopy. (C) Expression of E-cadherin and vimentin in cells treated with DMSO Ctrl or UO126 was analyzed by Western blot analysis. (D) MCF-7 Snail were treated for 6 h and 24 h with DMSO Ctrl, UO126 and UO126+MG132. Cell lysates were analyzed by Western blot analysis. (E) Migration and (F) adhesion assays was performed on MCF-7 Neo and MCF-7 Snail cells treated with DMSO Ctrl or UO126. β-actin was utilized as a loading control for Western blot analysis; DAPI was used to identify the nuclei in immunofluorescence analyses. Maginification 40X. Statistical Analysis was done using ANOVA and Tukey's Multiple Comparison as Post Hoc (**p≤0.01, ***p≤0.001). Values were expressed as mean ± S.E.M (N = 3). Results are representative of at least three independent experiments.

History