Influence of the heat treatment and EDTA on the haemagglutinating activity of the Eutirucallin.

<p><b>A.</b> Eutirucallin (1.8 µg) was incubated for 20 min at 25, 35, 45, 55, 65, 75, 85 and 95°C. After 60 min of sedimentation at room temperature the haemagglutinating activity was evaluated. <b>B.</b> The Eutirucallin (1.8 µg) was incubated with EDTA in the concentrations of 10, 50 and 100 mM, and kept for 18 h at 4°C. Following to that the haemagglutinating activity was evaluated. Human erythrocytes A+ at 2% were used in both assays. The positive haemagglutination was indicated by the formation of a uniform coating of red blood cells in the surface of the U plates, and its absence was determined by the sedimentation of the cells in the bottom of the plates.</p>