Influence of Manumycin A on protein and mRNA expression levels of IL-17/IL-22/IL-23, GM-CSF, and IFN-γ.

Effect of Manumycin A treatment (10 µM) on the (A) mRNA expression levels of IL-17, IL-22, IL-23, GM-CSF, and IFN-γ in K-ras positive colorectal cancer patients as performed by RT-PCR analysis. Patient stage D colon cells were treated with Manumycin A (10µM) for 24 hr, before RT-PCR. 5 µg of total RNA was isolated from treated colon cells. Values represent the mean ± SD of three independent experiments. (B) protein expression levels of IL-17, IL-22, IL-23, GM-CSF, and IFN-γ in K-ras positive colorectal cancer patients as performed by Western Blot analysis. Patient stage D colon cells were treated with Manumycin A (10µM) for 24 hr, before protein quantification. Values represent the mean ± SD of three independent experiments.